SIGMAFAST 3,3′-Diaminobenzidine tablets

tablet, To prepare 15 mL

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water: 1 tablet/5 mL

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Developed for use in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. DAB is the immunohistology substrate of choice as it produces an intense brown-black stain which is resistant to alcohol. Slides stained with DAB may be dehydrated, mounted in resinous media, and stored for future reference. SIGMAFAST DAB Tablets require no additional buffers or steps to prepare an active substrate solution. Tablets are individually packaged in foil packets


Each tablet set dissolved in 15 mL deionized water yields a ready-to-use buffered solution containing DAB and urea hydrogen peroxide.

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SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC


Exclamation markHealth hazard

Signal Word


Target Organs

Respiratory system


UN 3316 9

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

E N Scully et al.
Behavioural brain research, 393, 112775-112775 (2020-06-23)
In both humans and animals, biological differences between males and females has long been a topic of research. In songbirds, sexual dimorphisms can be seen in many species' plumage and heard in some species' songs. However, not all songbirds have...
F Gualtieri et al.
Scientific reports, 9(1), 7129-7129 (2019-05-11)
In the mammalian brain, adult hippocampal neurogenesis (AHN) is suppressed by chronic stress, primarily at the ventral pole of the hippocampus. Based upon anatomy, we hypothesise that the caudal pole of the avian Hippocampal Formation (HF) presents a homologous subregion....
Jari Willing et al.
Developmental psychobiology, 59(5), 583-589 (2017-06-01)
Adolescence is associated with continued maturation of the cerebral cortex, particularly the medial prefrontal cortex (mPFC). We have previously documented pruning in the number of neurons, dendrites, and synapses in the rat mPFC from preadolescence to adulthood, with the period...
Silvia Gasparini et al.
Brain structure & function, 224(1), 387-417 (2018-10-22)
Sodium deficiency elevates aldosterone, which in addition to epithelial tissues acts on the brain to promote dysphoric symptoms and salt intake. Aldosterone boosts the activity of neurons that express 11-beta-hydroxysteroid dehydrogenase type 2 (HSD2), a hallmark of aldosterone-sensitive cells. To...
Monika Verma et al.
Toxicon : official journal of the International Society on Toxinology, 139, 58-65 (2017-09-19)
Anthrax associated causalities in humans and animals are implicated mainly due to the action of two exotoxins that are secreted by the bacterium Bacillus antharcis during the infection. These exotoxins comprise of three protein components namely protective antigen (PA), lethal...
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.
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Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
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