Skip to Content
MilliporeSigma
All Photos(2)

Key Documents

D2813

Sigma-Aldrich

4,5-Diaminofluorescein diacetate

≥98% purity (HPLC), lyophilized powder

Synonym(s):

DAF-2 DA

Sign Into View Organizational & Contract Pricing


About This Item

Empirical Formula (Hill Notation):
C24H18N2O7
CAS Number:
Molecular Weight:
446.41
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

4,5-Diaminofluorescein diacetate, ≥98% (HPLC)

Quality Level

assay

≥98% (HPLC)

form

lyophilized powder

color

colorless to white

solubility

DMSO: <2 mg/mL
H2O: <2 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

shipped in

wet ice

storage temp.

2-8°C

SMILES string

O=C1OC2(C3=C(OC4=C2C=CC(OC(C)=O)=C4)C=C(OC(C)=O)C=C3)C5=C1C=C(N)C(N)=C5

InChI

1S/C24H18N2O7/c1-11(27)30-13-3-5-16-21(7-13)32-22-8-14(31-12(2)28)4-6-17(22)24(16)18-10-20(26)19(25)9-15(18)23(29)33-24/h3-10H,25-26H2,1-2H3

InChI key

PTSUYDXEEKDBQU-UHFFFAOYSA-N

Application

4,5-Diaminofluorescein diacetate (DAF-2 DA) has been used as a fluorescent dye for NO (nitric oxide) detection.
Cell permeable fluorescent detector of nitric oxide in living cells.

Legal Information

DAF-2 is manufactured by Daiichi Pure Chemicals Co., LTD.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Vitamin C prevents the endothelial dysfunction induced by acute ethanol intake.
Hipolito UV, et al.
Life Sciences, 141, 99-107 (2015)
Graphene Oxides Show Angiogenic Properties.
Mukherjee S, et al.
Advanced Helathcare Materials, 4, 1722-1732 (2015)
Willson Kwok et al.
Shock (Augusta, Ga.), 42(6), 554-561 (2014-09-23)
The purpose of this study is to understand the role of rho-kinase (ROCK-2) in the regulation of liver microcirculation after inflammatory stress. Endothelin-1 (ET-1)-induced nitric oxide (NO) is essential in the regulation of blood flow in hepatic sinusoids. Lipopolysaccharide (LPS)
Francis O'Neill et al.
Atherosclerosis, 241(2), 588-594 (2015-06-27)
Recent failures of clinical trials promoting HDL-elevating therapies have prompted research groups to focus on its functional activity in disease. Endothelial effects of HDL can be measured with in vitro cell assays. The reproducibility and biological relevance of these assays have
Daniel R Gonzalez et al.
American journal of physiology. Heart and circulatory physiology, 307(5), H710-H721 (2014-07-13)
Duchenne muscular dystrophy may affect cardiac muscle, producing a dystrophic cardiomyopathy in humans and the mdx mouse. We tested the hypothesis that oxidative stress participates in disrupting calcium handling and contractility in the mdx mouse with established cardiomyopathy. We found

Articles

Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service