CS1000
Superoxide Anion Assay Kit
sufficient for 100 assays (96 well plates)
Synonym(s):
Superoxide Detection Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Application
The Superoxide Anion assay kit provides a useful qualitative cell-based assay for the measurement of O2− anion status in cells. The kit can be used to test changes in superoxide anion levels following oxidative stress directly in whole cells. In addition, the kit can be also used for the detection of superoxide dismutase (SOD) activity. This activity was tested on erythrocytes, leucocytes, and plasma.
Biochem/physiol Actions
The superoxide anion (O2-) is a short-lived radical generated by the addition of an electron to oxygen. It is formed in response to environmental factors such as UV light, cigarette smoke, environmental pollutants, and γ-radiation, or by oxidases like xanthine oxidase or NADPH oxidase. Once formed, O2- attacks cellular components causing damage to lipids, proteins, and DNA. This can initiate numerous diseases, including cancer, atherosclerosis, rheumatoid arthritis, diabetes, liver damage, and central nervous system disorders.
Analysis Note
The kit method is based on the oxidation of luminol by superoxide anions resulting in the formation of chemiluminescence light. This method utilizes a specific, non-toxic enhancer that amplifies the chemiluminescent signal.
Kit Components Only
Product No.
Description
- Assay Buffer 25 mL
- Assay Medium 20 mL
- Enhancer Solution, 40 mM 500 μL
- Luminol Solution 500 μL
- Xanthine Solution 500 μL
- Xanthine Oxidase .5 U
- Superoxide Dismutase from bovine liver, 4 units/μL 400 U
Storage Class
10 - Combustible liquids
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