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CS0760

Sigma-Aldrich

Isolated Mitochondria Staining Kit

1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1,000 reactions (using 96 multiwell plates)

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EC Number:
217-896-6
NACRES:
NA.32

Quality Level

200

usage

 kit sufficient for 1,000 reactions (using 96 multiwell plates)
 kit sufficient for 50 reactions (in a 2 mL cuvette)

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

flow cytometry: suitable
protein staining: suitable

fluorescence

λex 490 nm; λem 590 nm (JC-1 dye)

application(s)

cell analysis
detection

detection method

fluorometric

shipped in

dry ice

storage temp.

−20°C

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Isolated Mitochondria Staining Kit 1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1,000 reactions (using 96 multiwell plates)

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Isolated Mitochondria Staining Kit

Citrate Synthase Assay Kit 1 kit sufficient for 100 reactions (using a 1 ml cuvette), 1 kit sufficient for 480 reactions (using 96 multiwell plates)

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Flow Cytometry Kit for Apoptosis

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packaging

pkg of 1 kit

packaging

-

packaging

pkg of 1 kit

packaging

pkg of 1 kit

storage condition

dry at room temperature

storage condition

-

storage condition

dry at room temperature

storage condition

-

technique(s)

flow cytometry: suitable, protein staining: suitable

technique(s)

-

technique(s)

flow cytometry: suitable, protein staining: suitable

technique(s)

flow cytometry: suitable

fluorescence

λex 490 nm; λem 590 nm (JC-1 dye)

fluorescence

-

fluorescence

λex 490 nm; λem 530 nm (green) (JC-1 monomers), λex 525 nm; λem 590 nm (red) (JC-1 aggregates)

fluorescence

-

detection method

fluorometric

detection method

-

detection method

fluorometric

detection method

fluorometric

Application

Isolated Mitochondria Staining Kit has been used to assess mitochondrial inner membrane potential.

Features and Benefits

  • Fast, simple, and convenient method for staining isolated mitochondria and for assaying mitochondria intactness
  • Contains all the reagents required for the detection of changes in mitochondrial inner-membrane electrochemical potential
  • Contains the antibiotic Valinomycin that permeabilizes the mitochondrial membrane, and thus, dissipates the mitochondrial electrochemical potential. Valinomycin can be used as a control agent that prevents JC-1 aggregation

Principle

The kit is based on mitochondria staining using the JC-1 dye. In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) does not allow the accumulation of JC-1 dye in the mitochondria. The dye is detected using a fluorimeter or fluorescence microscope.

Analysis Note

The kit measures mitochondria intactness and detects events that dissipate the mitochondrial membrane potential.

Kit Components Only

Product No.
Description
  • DMSO 1 mL
  • JC-1 Assay Buffer 5X 25 mL
  • JC-1 Stain 25 μg
  • Valinomycin Ready Made 100 μL

Pictograms

Skull and crossbones
GHS06

Signal Word

Danger

Hazard Statements

H300 + H310

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 1 Oral

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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25G
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Subcellular fractionation of mitochondria.
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