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CS0760
Isolated Mitochondria Staining Kit
1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1,000 reactions (using 96 multiwell plates)
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EC Number:
NACRES:
NA.32
Quality Level
usage
kit sufficient for 1,000 reactions (using 96 multiwell plates)
kit sufficient for 50 reactions (in a 2 mL cuvette)
packaging
pkg of 1 kit
storage condition
dry at room temperature
technique(s)
flow cytometry: suitable
protein staining: suitable
fluorescence
λex 490 nm; λem 590 nm (JC-1 dye)
application(s)
cell analysis
detection
detection method
fluorometric
shipped in
dry ice
storage temp.
−20°C
Application
Isolated Mitochondria Staining Kit has been used to assess mitochondrial inner membrane potential.
Features and Benefits
- Fast, simple, and convenient method for staining isolated mitochondria and for assaying mitochondria intactness
- Contains all the reagents required for the detection of changes in mitochondrial inner-membrane electrochemical potential
- Contains the antibiotic Valinomycin that permeabilizes the mitochondrial membrane, and thus, dissipates the mitochondrial electrochemical potential. Valinomycin can be used as a control agent that prevents JC-1 aggregation
Principle
The kit is based on mitochondria staining using the JC-1 dye. In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) does not allow the accumulation of JC-1 dye in the mitochondria. The dye is detected using a fluorimeter or fluorescence microscope.
Analysis Note
The kit measures mitochondria intactness and detects events that dissipate the mitochondrial membrane potential.
Kit Components Only
Product No.
Description
- DMSO 1 mL
- JC-1 Assay Buffer 5X 25 mL
- JC-1 Stain 25 μg
- Valinomycin Ready Made 100 μL
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 1 Dermal - Acute Tox. 1 Oral
Storage Class Code
6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificate of Analysis
Enter Lot Number to search for Certificate of Analysis (COA).
Certificate of Origin
Enter Lot Number to search for Certificate of Origin (COO).
Product Information Sheet
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