MilliporeSigma
All Photos(1)

My-La CD8+

95051033

biological source

human lymph

growth mode

Suspension

karyotype

Not specified

morphology

Lymphoblastoid

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human Caucasian T Lymphocyte, mycosis fungoides.

Cell Line Description

The cell line My-La, CD8+, also known as My-La, marker chromosome was established from a plaque biopsy of an 82 year old Caucasian patient with mycosis fungoides stage II. Together with My-La, CD8+ a second cell line has been established (My-La, CD4+ Sigma Catalogue number. 95051032) by including IL-2 and IL-4 in the culture medium. My-La, CD8+ shows characteristic chromosome markers, express ß-13 of the T-cell receptor and are double homozygous for HLA-A1.B8. Phenotypically a weak staining was observed for CD2, CD3, CD8, CD25, CD71 and TCR-1 α / ß. There has been no evidence of HTLV-I infection in the cell line and serum from the patient did not demonstrate antibodies against HTLV-I and HTLV-II. My-La, CD8+ cells show a low viability after resuscitation from frozen and a long lag time phase until exponential growth is reached.

Application

Study of antigen-independent proliferation of T- cell lyphoma from mycosis fungoides patient.

Culture Medium

RPMI 1640 + 2mM Glutamine + 100 u/ml IL-2 + 10% human AB seum

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37°C. Split only 1:2 as maximum dilution: freeze in growth medium plus 10% DMSO, low viability on resuscitation. Use of human serum produces high levels of debris in cultures.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service