All Photos(1)
biological source
human brain
growth mode
Adherent
karyotype
2n = 87-91, hypotetraploid
morphology
Glial
products
Not specified
receptors
Epidermal growth factor (EGF) R
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Human glioblastoma
Cell Line Description
Derived from an adult female with glioblastoma multiforme treated with local brain irradiation and multi drug chemotherapy. By flow cytometry these cells were almost 100% positive for MHC Class I.
Application
Tumourigenicity studies
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 9
D16S539: 12
D5S818: 12,13
D7S820: 11
THO1: 8
TPOX: 8
vWA: 15,16
CSF1PO: 10,11
D13S317: 9
D16S539: 12
D5S818: 12,13
D7S820: 11
THO1: 8
TPOX: 8
vWA: 15,16
Culture Medium
RPMI 1640 + 2mM Glutamine + 1% HT + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificate of Analysis
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Certificate of Origin
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