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COLO 741

93052621

biological source

human colon (pelvic wall metastasis)

growth mode

Adherent

karyotype

Not specified

morphology

Fibroblast-like

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

metastasis

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human colon carcinoma

Cell Line Description

Derived from a pelvic wall metastasis of a 69 year old female with colon carcinoma.

Application

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 9,11
D16S539: 11,12
D5S818: 13
D7S820: 12
THO1: 7,9
TPOX: 8,11
vWA: 17,18

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificate of Analysis

Certificate of Origin

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