All Photos(1)

A15

93040110

biological source

rat brain

growth mode

Adherent

karyotype

Modal no. 42

morphology

Not specified

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Rat, BDIX, glioma

Cell Line Description

A renamed subculture of the rat gliomal line 1A2(R) - 93040129. A15 is tumourigenic in BDIX rats.

Application

Tumourigenicity studies

Culture Medium

DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:50 to 1:100 i.e. seeding at 2-4x10,000 cells/cm2 flask using 0.25% trypsin/EDTA; 10-15% CO2; 37°C. Cells can reach up to 4x1,000,000 cells per 25cm2 flask.

Other Notes

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Certificate of Analysis

Certificate of Origin

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