MilliporeSigma
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U-2 OS Cell Line human

osteosarcoma, 92022711

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Synonym(s):
U2OS Cells, U20S Cells, U-2OS Cells, U2-OS Cells

biological source

human bone

growth mode

Adherent

karyotype

Not specified

morphology

Not specified

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human Osteosarcoma

Cell Line Description

Cell line derived in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl.

Application

U-2 OS has been used to study:
  • the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA)
  • the interaction of human single-stranded DNA binding protein 1 (hSSB1) with bloom syndrome protein helicase (BLM helicase)
  • calcium-mediated actin reset (CaAR) in response to physiological changes

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 13
D13S317: 13
D16S539: 11,12
D5S818: 11
D7S820: 11,12
THO1: 6,9.3
TPOX: 11,12
vWA: 14,18

Culture Medium

McCoy′s 5a medium with 1.5 mM Glutamine; 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split subconfluent 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new culture flasks.

Other Notes

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Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Articles

Cell Cycle Analysis Using a Nucleoside Triphosphate (NTP) Transporter Molecule for Rapid DNA Labeling in Living Cells

Regulation of the cell cycle involves processes crucial to the survival of a cell, including the detection and repair of genetic damage as well as the prevention of uncontrolled cell division associated with cancer. The cell cycle is a four-stage process in which the cell 1) increases in size (G1-stage), 2) copies its DNA (synthesis, S-stage), 3) prepares to divide (G2-stage), and 4) divides (mitosis, M-stage). Due to their anionic nature, nucleoside triphosphates (NTPs), the building blocks of both RNA and DNA, do not permeate cell membranes.

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