All Photos(1)
biological source
human colon
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Elevated levels of cystic fibrosis transmembrane conductance regulator (CFTR) protein
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Human Caucasian colon adenocarcinoma
Cell Line Description
A subclone of the parent HT29-18 differentiated to specific cell types for studies on cystic fibrosis.
Culture Medium
DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 44mM Sodium Bicarbonate (NaHCO3) + 10mg/l Transferrin + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:8, i.e. seeding at 1-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificate of Analysis
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Certificate of Origin
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