Cell Line Origin
Human Caucasian colon adenocarcinoma
Cell Line Description
A subclone of the parent HT29-18 differentiated to specific cell types for studies on cystic fibrosis.
DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 44mM Sodium Bicarbonate (NaHCO3) + 10mg/l Transferrin + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:8, i.e. seeding at 1-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
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