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HT29 gluc C1

92012401

biological source

human colon

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

Elevated levels of cystic fibrosis transmembrane conductance regulator (CFTR) protein

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human Caucasian colon adenocarcinoma

Cell Line Description

A subclone of the parent HT29-18 differentiated to specific cell types for studies on cystic fibrosis.

Culture Medium

DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 44mM Sodium Bicarbonate (NaHCO3) + 10mg/l Transferrin + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:8, i.e. seeding at 1-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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Certificate of Analysis

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Certificate of Origin

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