All Photos(1)

70Z/3

91092002

biological source

mouse blood

growth mode

Suspension

karyotype

Not specified

morphology

Lymphoblastoid

products

Cytoplasmic Immunoglobulin

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse pre-B lymphocyte

Cell Line Description

Derived from a methyl nitrosourea-induced tumour in a (C57BL/6XDBA/2) F1 mouse. This line is positive for cytoplasmic Ig and negative for surface membrane Ig and TdT.

Culture Medium

RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 2-5x100,000 cells/ml; 5% CO2; 37°C.

Other Notes

Depositor and originator: Dr P Kincade, Oklahoma Medical Research Foundation
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Certificate of Analysis

Certificate of Origin

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