Recommended Products
biological source
human blood
growth mode
Suspension
karyotype
Modal no. 45
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Human Caucasian promyelocytic leukaemia
Cell Line Description
HL60 induced to mature into neutrophils with 1.75% DMSO after treating with SV40. Fails to express SV40 specific T Ag suggesting that HL60 M4 represents expansion of sub-clones of HL60 with inherent restricted capacity for neutrophil differentiation.
Application
Differentiation studies
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml. Do not allow the culture medium to become acidic or the cells will differentiate; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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