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biological source
Mouse x Rat Hybridoma nerve
growth mode
Adherent
karyotype
Not specified
morphology
Neuronal
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse neuroblastoma x Rat glioma hybrid
Cell Line Description
Neuroblastoma x glioma hybrid was formed by Sendai virus-induced fusion of the mouse neuroblastoma clone N18TG-2 and the rat glioma clone C6 BV-1.
Application
Hybridisation, fusion partner and virus studies: Sendai virus
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 seeding at 2x100,000 cells/cm2 . Cells adhere lightly, detach by gently knocking the flask do not use trypsin. 5% CO2; 37°C. The cells do produce large amounts of CO2, media changes may be necessary.
Other Notes
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Certificates of Analysis (COA)
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Articles
Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines at sigmaaldrich.com
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