Cell Line Origin
Fish Fat Head minnow
Cell Line Description
Derived from tissue posterior to the anus of normal adult minnows. Reported to be capable of growth over a wide temperature range from 4°C-36°C with maximum growth at 34°C. Support growth of the virus of infectious pancreatic necrosis (IPN), cat fish tumour virus (CTV) and Granoff frog virus (FV1). ECHO-II will multiply in FHM but the cytopathic effect is not extensive.
Virus studies: Inf. pacr. necrotic virus, cat fish tumour, and Granoff′s frog viruses
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) +10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:3; 34°C. Dislodge cells by agitation and scraping. Cells are reported to multiply over a wide range of temperatures 0°-36°C, with maximum growth at about 34°C. It is recommended that cultures are grown at 34
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.