MilliporeSigma
All Photos(1)

LS180

87021202

All Photos(1)

biological source

human colon

growth mode

Adherent

karyotype

Modal no. 45 (2n=46)

morphology

Epithelial-like

products

Carcinoembryonic antigen (CEA), Mucin

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Compare Similar Items

View Full Comparison

1 of 4

This Item
CB_94120801CB_87060401CB_86032004
LS180 87021202

LS180

LS 123 94120801

LS 123

LS174T 87060401

LS174T

L929S 86032004

L929S

biological source

human colon

biological source

human colon

biological source

human colon

biological source

mouse adipose tissue

growth mode

Adherent

growth mode

Adherent

growth mode

Adherent

growth mode

Suspension

karyotype

Modal no. 45 (2n=46)

karyotype

Modal no. 63 (2n=46), hypotriploid

karyotype

Modal no. 45 (2n

karyotype

Not specified

morphology

Epithelial-like

morphology

Epithelial

morphology

Epithelial

morphology

Not specified

products

Carcinoembryonic antigen (CEA), Mucin

products

Carcinoembryonic antigen (CEA)

products

Not specified

products

Not specified

receptors

Not specified

receptors

Not specified

receptors

Not specified

receptors

Not specified

Cell Line Origin

Human Caucasian colon adenocarcinoma

Cell Line Description

Derived from a 58 year old female Caucasian with Dukes type B adenocarcinoma of the colon. The cells secrete CEA and mucin and are tumorigenic in nude mice. LS174T (Sigma Catalogue number. 87060401) was derived from the same patient. The cells have not been exposed to trypsin.

Application

Tumourigenicity studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm2 by scraping; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

(enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service