Product has lost contact inhibition
Cell Line Origin
Mouse Swiss Albino embryo fibroblast
Cell Line Description
Derived from Swiss mouse embryos this cell line secretes collagen and hyaluronic acid. Unlike 3T3, the 3T6 line is not contact inhibited.
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 5x1,000 to 3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
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