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biological source
Mouse x Mouse Hybridoma
ImmunoDon
CAF1 mouse spleen
Myeloma
P3X63Ag8
Reactivity
Anti-CD3, human
technique(s)
cell culture | mammalian: suitable
isotype
IgG2a
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse P3X63Ag8 x Mouse CAF1 spleen
Cell Line Description
The antibody is directed against the CD3 antigen on human T cells.
Immunogen
Human peripheral blood lymphocytes
Culture Medium
IDMEM + 2mM glutamine + 20% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cells may be slow to grow upon resuscitation and may take up to 7 days before they are ready for subculture.
Other Notes
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Certificates of Analysis (COA)
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