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biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
Myeloma
P3X63Ag8
Reactivity
Anti-IgD, mouse
technique(s)
cell culture | mammalian: suitable
isotype
IgM
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse P3X63Ag8 x Mouse BALB/c spleen
Cell Line Description
The antibody is directed against ′IgD-like′ molecules of mice with IgB haplotype. The target antigen has a molecular weight of 185kDa and is found on a high proportion of mature lymphoid cells, particularly on cells from Peyer′s patches. It is not detected on thymus cells.
Immunogen
Two grafts of full thickness skin from B10 mice followed by B10 spleen cells
Culture Medium
DMEM + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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