53B3

Application: Fusion partner, 85062501

• UNSPSC Code: 41106514

Properties

• biological source: Mouse x Bovine Heterohybridoma
• ImmunoDon: Jersey calf lymph node
• Myeloma: 53B3 (mouse x bovine heteromyeloma)
• Reactivity: Application: Fusion partner
• technique(s): cell culture | mammalian: suitable
• isotype: Not specified
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse x Bovine 53B3 x Jersey calf lymph node

Cell Line Description

This HAT sensitive line can be used as a fusion partner in the production of heterohybridomas.

Immunogen

Sheep red blood cells

Application

Fusion partner

Culture Medium

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Other Notes

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