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3T3 Swiss Albino


biological source

mouse embryo

growth mode



2n = 40; hypertriploid




Not specified


Not specified


cell culture | mammalian: suitable

shipped in

dry ice

storage temp.


Related Categories

General description

Product has lost contact inhibition

Cell Line Origin

Mouse Swiss Albino embryo fibroblast

Cell Line Description

Evolved under conditions favouring retention of the property of contact inhibition of cell proliferation; they exhibit much more contact inhibition than diploid fibroblasts freshly plated in culture. Readily transformed by polyoma and SV40 viruses resulting in reduced sensitivity to contact inhibition. Cells have now lost their contact inhibition.


Contact inhibition and virus studies: polyoma and SV40 viruses

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 5x1,000 to 1x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificate of Analysis

Certificate of Origin

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