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biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
Myeloma
P3-NS1/1-Ag4.1
Reactivity
Human ′A′ erythrocytes
technique(s)
cell culture | mammalian: suitable
isotype
IgM
shipped in
dry ice
storage temp.
−196°C
Cell Line Description
The W6/1 hybridoma cell line secretes monoclonal IgM antibodies reactive with a polymorphic determinant of human erythrocytes which correlated with blood group A.
Immunogen
Human tonsil membrane
Application
Human erythrocyte marker.
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)
Subculture Routine
Seed at 5 x 104 cells/ml. Subculture when cells reach 5 x 105 -1 x 106 cells/ml (usually after 3-4 days), 5% CO2, 37°C. Start in 20% FBS when recovering from frozen.
Other Notes
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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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