MM138 Cell Line human
10092321, human skin, Epithelial
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About This Item
UNSPSC Code:
41106514
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product name
MM138 Cell Line human, 10092321
biological source
human skin
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human melanoma; from metastatic site - lymph node
Cell Line Description
Metastatic Melanoma (metastatic site: lymph node). Cell Bank Australia reference number: CBA1345.
Culture Medium
RPMI 1640 (with 2 mM L-Glutamine + 25 mM HEPES) + 10% fetal bovine serum (FBS).
Subculture Routine
Optimal split ratio 1:4, MM138 has a relatively slow doubling time of around 60 hours. It does not become confluent and will detach after 6-7 days. If split too hard the doubling time is greatly increased. (Seeding density 0.8 x104 cells/cm2). Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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