Mero-14

9100101, human lung, Spindle shaped cells

• Synonym(s): Mero14 Cells
• UNSPSC Code: 41106514

Properties

• product name: Mero-14, 09100101
• biological source: human lung
• growth mode: Adherent
• karyotype: Number of chromosomes 72-75
• morphology: Spindle shaped cells
• products: Cytokeratins 7, 8, 18 and 19. Epithelial membrane antigen (EMA)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure

Cell Line Description

Mero-14 was derived from the pleural effusion from a male patient aged >60 with a history of asbestos exposure. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Application

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 12,13
D5S818: 9
D7S820: 10,11
THO1: 8
TPOX: 9,11
vWA: 15,18

Culture Medium

Hams F10 + 15% FCS + 2mM Glutamine

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Population doubling approx 24hrs

Other Notes

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