All Photos(1)



biological source

rat brain

growth mode



Not specified




Not specified


Vasopressin V1a, Angiotensin II, Endothelin 1, Bradykinin, Serotonin


cell culture | mammalian: suitable

shipped in

dry ice

storage temp.


Cell Line Origin

Rat Choroid Plexus

Cell Line Description

The choroid plexus of adult Wistar Rats were dissected from the fourth ventricle to give a primary culture. This primary culture was then immortalized by transfection with the TSori minus adenovirus (a replication deficient adenovirus containing SV40 large T antigen). Sub-cloning of the immortalised cells was performed by selecting for clones which exhibited calcium ion release in response to vasopressin, angiotensin, bradykinin, serotonin or endothelin stimulation. The sub-clone which exhibited the largest response to all five hormones was selected and sub-cultured on to become RCP.


Toxicology, cellular signalling and pharmacology studies

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C

Other Notes

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Certificate of Analysis

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Certificate of Origin

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