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RCP

00031626

biological source

rat brain

growth mode

Adherent

karyotype

Not specified

morphology

Endothelial

products

Not specified

receptors

Vasopressin V1a, Angiotensin II, Endothelin 1, Bradykinin, Serotonin

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Rat Choroid Plexus

Cell Line Description

The choroid plexus of adult Wistar Rats were dissected from the fourth ventricle to give a primary culture. This primary culture was then immortalized by transfection with the TSori minus adenovirus (a replication deficient adenovirus containing SV40 large T antigen). Sub-cloning of the immortalised cells was performed by selecting for clones which exhibited calcium ion release in response to vasopressin, angiotensin, bradykinin, serotonin or endothelin stimulation. The sub-clone which exhibited the largest response to all five hormones was selected and sub-cultured on to become RCP.

Application

Toxicology, cellular signalling and pharmacology studies

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C

Other Notes

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Certificate of Analysis

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Certificate of Origin

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