MilliporeSigma
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CB0500

Sigma-Aldrich

CelLytic B Plus Kit

For bacterial lysis

Synonym(s):
Lysis and protein extraction kit
EC Number:
NACRES:
NA.56

Quality Level

usage

1 kit sufficient for 50 g fresh or frozen cell paste lysis.

shipped in

wet ice

storage temp.

−20°C

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General description

The CelLytic B Plus Kit is designed to efficiently lyse cells and extract proteins from both Gram negative, and difficult to lyse, Gram positive bacteria. This is accomplished using the standard CelLytic B, a proprietary non-ionic detergent in concert with lysozyme, Benzonase®, and protease inhibitors. This complete kit takes the guesswork out of protein extraction from a variety of bacterial species.

Application

This kit is compatible with FLAG®, HIS-Select®, and glutathione S-transferase based affinity chroma­tography protein purification systems. The detergent included in the CelLytic B Lysis Reagent can be removed from the protein by dialysis or ammonium sulfate precipitation, if necessary. The final purity of the recombinant product is usually higher than that obtained from traditional extraction methods. This is because the crude protein solution containing the non-ionic detergent eliminates much of the non-specific binding that occurs during chromatography. The mild detergent does not interfere with many enzyme assays or protein assays.

Features and Benefits

  • Lyse Gram positive and Gram negative bacteria
  • More efficient than sonication
  • Compatible with affinity purification
  • Isolate inclusion bodies for subsequent solubilization
  • Non-denaturing cell lysis preserves protein function

Other Notes

The CelLytic B Plus Kit contains all of the reagents and chemicals necessary to lyse both Gram-negative and Gram-positive bacteria. This kit also includes protease inhibitors to help prevent the proteolytic breakdown of proteins.

Legal Information

Benzonase® Nuclease is manufactured by Merck KGgA, Darmstadt, Germany and its affiliates. Nycomed Pharma A/S (Denmark) claims worldwide patent rights to Benzonase Nuclease, which are licensed exclusively to Merck KGaA, Darmstadt, Germany.
Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
CelLytic is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS

  • B7435CelLytic B Cell Lysis Reagent, For bacterial cell lysis, standard strength

  • E1014Benzonase® Nuclease, ≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

  • P8849Protease Inhibitor Cocktail, for use in purification of Histidine-tagged proteins, DMSO solution

  • L3790Lysozyme from chicken egg white, 10 mg/mL

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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