All Photos(1)



Caspase 3 Assay Kit, Colorimetric



sufficient for 1,000 multiwell tests (in 96-well multiwell plates)
sufficient for 100 standard tests (1 ml)

Quality Level

shipped in

dry ice

storage temp.


Gene Information

human ... CASP3(836)

General description

Caspase 3 Colorimetric Assay Kit provides the reagents needed for a quick and efficient detection of caspase 3 activity in cell lysates and in purified preparations of caspase 3.

The Caspase 3 Colorimetric Assay Kit is based on the hydrolysis of acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA) by caspase 3, resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline is detected at 405 nm (εmM=10.5). The concentration of the pNA released from the substrate is calculated from either the absorbance values at 405 nm or from a calibration curve prepared with pNA standards (pNA standard included with the kit).


The assay can be performed in 1 mL volume and measured using a spectrophotometer or in 100 ìl volume in a 96 well plate using an ELISA reader.

Unit Definition

One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD-pNA per min at pH 7.4 at 25 °C.

Other Notes

Use 17 megohm water only

Kit Components Only

Product No.

  • Water (17-18 megaOhm) 125 mL

Kit Components Also Available Separately

Product No.

  • A0835Ac-DEVD-CHO inhibitor .5 mg

  • A2559Ac-DEVD-pNA substrate 15 mg

  • p-Nitroaniline standard 1 mg


Skull and crossbonesHealth hazard

Signal Word


Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 3 - STOT RE 2

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects



Flash Point(F)

415.4 °F - closed cup

Flash Point(C)

213 °C - closed cup

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service