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C8977

Sigma-Aldrich

Monoclonal Anti-CREB antibody produced in rabbit

clone 48H2, purified immunoglobulin, buffered aqueous glycerol solution

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About This Item

MDL number:
MFCD01092316
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

48H2, monoclonal

form

buffered aqueous glycerol solution

mol wt

antigen 43 kDa

species reactivity

rat, human, monkey, mouse

technique(s)

ChIP: 1:50
flow cytometry: 1:400
immunocytochemistry: 1:800
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:6,400
immunohistochemistry (frozen sections): 1:6,400
immunoprecipitation (IP): 1:250
indirect immunofluorescence: 1:800
western blot: 1:1,000 using extracts from SK-N-MC cells treated with forskolin or FGF

isotype

IgG

UniProt accession no.

P16220

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CREB1(1385)
mouse ... Creb1(12912)
rat ... Creb1(81646)

Specificity

Detects endogenous levels of total CREB protein.

Immunogen

Recombinant protein specific to amino terminus of human CREB.

Physical form

Solution in 10 mM sodium HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/mL bovine serum albumin, and < 0.02% sodium azide with 50% glycerol.

Legal Information

Rabbit monoclonals produced using Epitomics® Technology, U.S.Patent No. 5,675,063.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Min Gyu Lee et al.
The Journal of biological chemistry, 278(42), 41047-41058 (2003-08-02)
A common signature of many cancers is a high glucose catabolic rate frequently dependent on the overexpression of Type II hexokinase (HKII), a mitochondrial bound enzyme that also suppresses cell death. As the tumor HKII promoter plays a significant role
CREB: a mediator of long-term memory from mollusks to mammals.
D A Frank et al.
Cell, 79(1), 5-8 (1994-10-07)
J C Yin et al.
Cell, 81(1), 107-115 (1995-04-07)
Genetic studies of memory formation in Drosophila have revealed that the formation of a protein synthesis-dependent long-term memory (LTM) requires multiple training sessions. LTM is blocked specifically by induced expression of a repressor isoform of the cAMP-responsive element-binding protein (CREB).
G A Gonzalez et al.
Cell, 59(4), 675-680 (1989-11-17)
In this paper, we demonstrate that phosphorylation of CREB at Ser-133 is induced 6-fold in vivo, following treatment of PC12 cells with forskolin. By contrast, no such induction was observed in the kinase A-deficient PC12 line A126-1B2 (A126). Using F9
R P Kwok et al.
Nature, 370(6486), 223-226 (1994-07-21)
The transcription factor CREB binds to a DNA element known as the cAMP-regulated enhancer (CRE). CREB is activated through phosphorylation by protein kinase A (PKA), but precisely how phosphorylation stimulates CREB function is unknown. One model is that phosphorylation may
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