MilliporeSigma
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C4606

Sigma-Aldrich

Anti-Calreticulin antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):
Anti-CRP55
MDL number:

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 50 kDa

species reactivity

human, canine

technique(s)

immunoprecipitation (IP): suitable using whole cell RIPA lysate of the human epitheloid carcinoma HeLa cell line
indirect immunofluorescence: 1:100 using 3% paraformaldehyde-fixed, 0.5% Triton X-100 treated, Madin Darby canine kidney MDCK cell line
microarray: suitable
western blot: 1:2,000 using whole cell RIPA lysate of the human epitheloid carcinoma HeLa cell line

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CALR(811)

General description

The gene for calreticulin (CALR) is located on the human chromosome 19p13.13.[75] The encoded protein is a predominantly conserved[35] calcium-binding chaperone, which is localized primarily to the lumen of the endoplasmic reticulum (ER).[10] Calreticulin comprises three domains, a globular N-domain, a proline-rich P-domain, and a highly acidic C-domain.[35]

Specificity

Anti-Calreticulin recognizes human and dog calreticulin (55-60 kDa).

Immunogen

synthetic peptide corresponding to the C-terminus of human calreticulin (amino acids 401-417).

Application

Anti-Calreticulin antibody produced in rabbit has been used in:
  • immunoblotting
[10][84]
  • immunofluorescence
  • [28]
  • immunocytochemistry{91
  • immunoprecipitation

  • Biochem/physiol Actions

    Calreticulin facilitates transient interaction with newly synthesized cellular and extracellular proteins for folding and assembling in the endoplasmic reticulum (ER) before its localization to the cytosol or cell surface. Calreticulin acts as a lectin-like chaperone binding oligosaccharide residues of newly synthesized N-linked glycoproteins and misfolded proteins.[95] It is believed to play a critical role in quality control processes during protein synthesis and folding and calcium (Ca2+) homeostasis.[35] Increased expression of calreticulin increases the Ca2+ storage capacity of the ER. It also appears to modulate store-operated Ca2+-influx and to alter Ca2+ transport by the sarcoplasmic/ER Ca2+-adenosine triphosphatase (ATPase) (SERCA).[95] Overexpression of calreticulin results in increased sensitivity of HeLa cells to drug-induced apoptosis. However, increased resistance to apoptosis has been observed in calreticulin-deficient cells.[98]

    Physical form

    Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

    Storage and Stability

    Store at -20 °C. For continuous use, the product maybe stored at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at-20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded ifnot used within 12 hours.

    Disclaimer

    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

    Storage Class Code

    12 - Non Combustible Liquids

    WGK

    nwg

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Certificate of Analysis

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    Certificate of Origin

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