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C4187

Sigma-Aldrich

Cyanoborohydride Coupling Buffer

NACRES:
NA.56

form

liquid

Quality Level

reaction suitability

reagent type: reductant

storage temp.

2-8°C

Application

Cyanoborohydride Coupling Buffer is used in affinity chromatography, protein chromatography, activated/functionalized matrices and synthetic reagents. Cyanoborohydride has been used to inform a safe and effective gene-transfer system targeting hepatocytes as well as to develop a method for targeted delivery of anticancer therapeutics to cancer cells in hypoxic areas.
A ready-to-use reagent used to couple amine ligands to aldehyde functional groups. The coupling buffer reaction is a reductive amination of the intermediate Schiff′s base to a stable C−N bond.
Cyanoborohydride Coupling Buffer has been used:
  • in coupling reactions between amines and glutaraldehyde
  • to reduce hydrazone bond to a stable hydrazide bond
  • as a component in oligonucleotide reaction mixture for coverslips functionalization

Biochem/physiol Actions

Cyanoborohydride Coupling Buffer is a reagent suitable for reductive amination processes, that contributes to transformation of simple alcohols into more complex amines. It is used in the conversion of Schiff base, by reducing it, to form a secondary amine without affecting aldehyde groups on the support.

Components

0.02 M sodium phosphate, pH 7.5, containing 0.2 M sodium chloride and 3.0 g/L sodium cyanoborohydride

Pictograms

Exclamation markEnvironment

Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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