C3117
Monoclonal Anti-Cy3/Cy5−Biotin antibody produced in mouse
~1 mg/mL, clone CY-96, purified immunoglobulin, buffered aqueous solution
Synonym(s):
Monoclonal Anti-Cy3/Cy5
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
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biological source
mouse
conjugate
biotin conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
CY-96, monoclonal
form
buffered aqueous solution
concentration
~1 mg/mL
technique(s)
direct ELISA: suitable
dot blot: suitable
immunocytochemistry: 1-2 μg/mL using chicken fibroblasts
microarray: suitable
isotype
IgG2a
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
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General description
Monoclonal Anti-Cy3/Cy5 (mouse IgG2a isotype), Biotin conjugate is derived from the CY-96 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a mixture of proteins labelled with cyanine 3 (Cy3) or Cy5. Cyanine dyes are ionized polymethine dyes.
Specificity
The antibody recognizes Cy3 and Cy5 conjugated to proteins.
Immunogen
mixture of proteins labeled with Cy3/Cy5.
Application
Monoclonal Anti-Cy3/Cy5 Biotin antibody produced in mouse has also been used in:
- enzyme linked immunosorbent assay (ELISA)
- immunocytochemistry
- dot blot assay
- protein microarrays
- immunofluorescenc
Monoclonal Anti-Cy3/Cy5-Biotin antibody produced in mouse was used to label miR-107 in human breast cancer cell lines by confocal microscopy.
Biochem/physiol Actions
Cy dyes are pH insensitive fluorophores that are used to label, DNA, RNA and proteins in a variety of biological assays.
Cyanine dyes are small, soluble in aqueous solution and has tolerance to dimethyl sulfoxide (DMSO). They are more photostable than fluorescein, has high molar extinction coefficients and favourable quantum yields. Monoclonal antibodies to cyanine 3 (Cy3) and/or Cy5 are an important tool for Cy3/Cy5 signal amplification in assays where biomolecules are labelled with Cy3 or Cy5.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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25th Anniversary Article: Design of Polymethine Dyes for All-Optical Switching Applications: Guidance from Theoretical and Computational Studies
Gieseking RL, et al.
Advanced Materials, 26(1), 68-84 (2014)
Quantitative profile of the uropathogenic Escherichia coli outer membrane proteome during growth in human urine
Alteri CJ and Mobley HLT
Infection and Immunity, 75(6), 2679-2688 (2007)
N Duval et al.
The Journal of cell biology, 118(3), 641-653 (1992-08-01)
We analyzed the production of Torpedo marmorata acetylcholinesterase (AChE) in transfected COS cells. We report that the presence of an aspartic acid at position 397, homologous to that observed in other cholinesterases and related enzymes (Krejci, E., N. Duval, A.
D M Mottola et al.
The Journal of pharmacology and experimental therapeutics, 262(1), 383-393 (1992-07-01)
The present work provides a detailed pharmacological characterization of dihydrexidine (DHX) (trans-10,11-dihydroxy- 5,6,6a,7,8,12b-hexahydrobenzo[a]phenanthridine), the first high-potency, full efficacy, bioavailable D1 dopamine receptor agonist. DHX represents a new conformationally rigid structural class of dopamine receptor ligands. It competes stereoselectively and potently
Pai-Sheng Chen et al.
The Journal of clinical investigation, 121(9), 3442-3455 (2011-08-16)
MicroRNAs (miRNAs) influence many biological processes, including cancer. They do so by posttranscriptionally repressing target mRNAs to which they have sequence complementarity. Although it has been postulated that miRNAs can regulate other miRNAs, this has never been shown experimentally to
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