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C-14080

Sigma-Aldrich

Human Cardiac Myocytes (HCM)

1 million cells per pellet

biological source

human heart (ventricles)

packaging

pkg of 1,000,000 cells

morphology

(myocytes)

application(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−20°C

Related Categories

Cell Line Origin

Heart

Application

Primary Human Cardiac Myocytes (HCM) are isolated from the ventricles of the adult heart. They are qualified for in vitro research on cardiac diseases and for pharmacological studies. Unlike freshly isolated rod-shaped myocytes, cultured HCM can be used for long-term experiments like investigating the long-term effects of cytokines, mechanical strain, or cell-cell interactions, as they are prepared according to a special protocol.Initially, the HCM act more like progenitor cells in that they are not yet fully differentiated. They express the markers of early stage differentiation such as GATA-4 and sarcomeric alpha-actin and have a high capacity for proliferation. When they are grown to confluency and cultivated for an extended period of time, the differentiation process begins. Markers of late differentiation (e.g. sarcomeric alpha-actinin, slow muscle myosin) are increased and the cells begin to form myotube-like structures.

Quality

Rigid quality control tests are performed for each lot of the corresponding cells. They are tested for cell viability, cell morphology, growth performance and for cell-type specific markers. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subculture Routine

Click here for more information.

Other Notes

The Cell Pellets can be stored indefinitely at -20°C, and for up to 1 month at 4°C. Note: The Cell Pellets will not freeze at -20°C. Always centrifuge product briefly before opening vial.

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Articles

Primary Human Cardiac Myocytes and Myocyte Growth Media

Primary human cardiac myocytes isolated from normal human ventricle tissue of the adult heart, plus optimized myocyte media. Protocols for handling, cell passaging, media, and product use.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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