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C-12921

Sigma-Aldrich

Human CD34+ Progenitor Cells (hCD34+-CB)

Isolated from cord blood, single donor, 100,000 cryopreserved cells

biological source

human umbilical cord blood

packaging

pkg of 100,000 cells

morphology

(progenitor)

application(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Cord Blood

Application

CD34 is a glycosylated transmembrane protein and represents a well-known marker for primitive blood- and bone marrow-derived progenitor cells, especially for hematopoietic and endothelial stem cells. Although the biological functions of CD34 are largely unknown, recent data suggest that CD34 is involved in maintenance of the progenitor cells in a phenotypically undifferentiated state. PromoCell offers CD34+ Progenitor Cells from the cord blood of healthy donors. The CD34+ Progenitor Cells contain two main cellular subpopulations, hematopoietic and endothelial progenitor cells. Therefore, CD34+ Progenitor Cells are suitable for a series of studies, e.g. directed differentiation into more committed types of blood cells and endothelial lineages.

Quality

Rigid quality control tests are performed for each lot of CD34+ Progenitor Cells. They are routinely characterized by flow cytometry analyzing the viability, cell size, and the presence of significant markers, i.e. CD34. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subculture Routine

Click here for more information.

Other Notes

Recommended Plating Density: 5000 cells per mlTested Markers: CD34 positive

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Articles

Human Hematopoietic Progenitor Cells (HPC) and Growth Media

Human Hematopoietic Progenitor Cells (HPC) and Growth Media

Protocols

In Vitro Expansion of CD34+/CD38- Human Hematopoietic Progenitor Cells in a Serum-free and Xeno-free Stem Cell Culture Media

In vitro culture of human hematopoietic stem cells in PromoCell’s serum-free/xeno-free HPC Expansion Medium DXF. Explore over 350 PromoCell products

Human Hematopoietic Progenitor Cells (HPC) and Growth Media

Human CD34+ progenitor cells and xeno-free, defined media for the expansion of hematopoietic progenitor cells. Protocols for handling, cell passaging, media, and product use.

Hematopoietic Stem Cell Culture Protocols

Step-by-step hematopoietic stem cell culture protocols for isolation, expansion and differentiation of CD34+ hematopoietic progenitor cells including CFU assays. It can be cultured under defined conditions designed either to promote self-renewal and increase the number of primitive cells.

In Vitro Differentiation of Human PBMC and CD34+ Derived Monocytes into Mature CD83+/CD14- Dendritic Cells

Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products on Sigma.com.

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