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Primary Human Cardiac Fibroblasts (HCF) are isolated from the ventricles of the adult heart and are provided in a cryopreserved format. They play a central role in the maintenance of the extracellular matrix in the normal heart and the synthesis of growth factors and cytokines. Under pathophysiological conditions, cardiac fibroblasts are involved in scar formation after myocardial infarction, cardiac fibrosis, and cardiac hypertrophy. HCF can be used to study such processes in vitro.
Rigid quality control tests are performed for each lot of Human Fibroblasts. They are tested for cell morphology, adherence rate and cell viability. Flow cytometric analyses of the fibroblast specific antigen CD90 are performed for each lot. Growth performance is tested through multiple passages up to 10 or 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
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Recommended Plating Density: 3500 - 7000 cells per cm2Passage After Thawing: P2Tested Markers: CD90 positive, Smooth muscle alpha-actin negative, slow muscle myosin negativeGuaranteed population doublings: >15