C-12300

Normal Human Dermal Fibroblasts (NHDF)

Juvenile foreskin, 500,000 cryopreserved cells

Properties

• biological source: human foreskin (juvenile dermis)
• packaging: pkg of 500,000 cells
• morphology: (fibroblast)
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

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Cell Line Origin

Skin

Application

Primary Normal Human Dermal Fibroblasts (NHDF) are isolated from the dermis of juvenile foreskin and are provided in a cryopreserved format. They can be used for wound healing studies and dermatological research to investigate diseases like scleroderma, fibrosarcoma, fibrosis, xeroderma pigmentosum, and histiocytoma. Moreover, fibroblasts are important for cancer research, tissue regeneration, and tissue engineering studies.Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Epidermal Melanocytes (NHEM) cultured in M2 medium from the same donor are available on request.

Quality

Rigid quality control tests are performed for each lot of Human Fibroblasts. They are tested for cell morphology, adherence rate and cell viability. Flow cytometric analyses of the fibroblast specific antigen CD90 are performed for each lot. Growth performance is tested through multiple passages up to 10 or 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subculture Routine

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Other Notes

Recommended Plating Density: 3500 - 7000 cells per cm²Passage After Thawing: P2Tested Markers: CD90 positiveGuaranteed population doublings: >15

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable