C111030

DMT-dC(bz) Phosphoramidite

configured for ABI

• Synonym(s): Cytidine, N-benzoyl-5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-deoxy-, 3′-[2-cyanoethyl bis(1-methylethyl)phosphoramidite], DMT-dC(bz) amidite, N-benzoyl-5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-deoxycytidine, 3′-[2-cyanoethyl N,N-?bis(1-methylethyl)phosphoramidite], N4-Benzoyl-5′-O-(4,4′-dimethoxytrityl)-2′-deoxycytidine-3′-O-[O-(2-cyanoethyl)-N,N′-diisopropylphosphoramidite]
• Empirical Formula (Hill Notation): C₄₆H₅₂N₅O₈P
• CAS Number: 102212-98-6
• Molecular Weight: 833.91
• MDL number: MFCD00036315
• UNSPSC Code: 41116107
• PubChem Substance ID: 329774848
• NACRES: NA.51

Properties

• biological source: non-animal source (no BSE/TSE risk)
• type: for DNA synthesis
• product line: Proligo Reagents
• assay: ≥99% (³¹P-NMR); ≥99.0% (reversed phase HPLC)
• form: powder or granules
• technique(s): oligo synthesis: suitable
• impurities: ≤0.3 wt. % water content (Karl Fischer)
• color: white to off-white
• λ: conforms (UV/VIS Identity)
• compatibility: configured for ABI
• nucleoside profile: base: deoxycytidine; base protecting group: benzoyl; 2' protecting group: none; 5' protecting group: DMT; deprotection: standard
• storage temp.: 2-8°C
• SMILES string: COc1ccc(cc1)C(OC[C@H]2O[C@H](C[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)N3C=CC(NC(=O)c4ccccc4)=NC3=O)(c5ccccc5)c6ccc(OC)cc6
• InChI: 1S/C46H52N5O8P/c1-32(2)51(33(3)4)60(57-29-13-27-47)59-40-30-43(50-28-26-42(49-45(50)53)48-44(52)34-14-9-7-10-15-34)58-41(40)31-56-46(35-16-11-8-12-17-35,36-18-22-38(54-5)23-19-36)37-20-24-39(55-6)25-21-37/h7-12,14-26,28,32-33,40-41,43H,13,29-31H2,1-6H3,(H,48,49,52,53)/t40-,41+,43+,60?/m0/s1
• InChI key: PGTNFMKLGRFZDX-SALLYJDFSA-N

Description

General description

Proligo′s DNA phosphoramidites lead to high-yield and high-quality oligonucleotides due to their high coupling efficiency. The deprotection step of automated oligonucleotide synthesis is integral to synthesis time and final product quality. Exocyclic amine functions are protected by a benzoyl group (dA(bz) and dC(bz)) or isobutyryl group (dG(ib))Key features of DNA phosphoramidites:

• Recommended cleavage and deprotection conditions are 8 hours at 55 °Cor 24 hours at room temperature using concentrated ammonia solution, for standard base-protected oligonucleotides
• The high coupling efficiency of Proligo′s DNA phosphoramidites leads to high-yield and high-quality oligonucleotides

The product belongs to ABI^™ synthesizers.

Legal Information

ABI is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable