form
solid
General description
Protein Extraction Reagent Type 1 is a highly effective protein solubilization and extraction reagent composed urea, Trizma® and CHAPS.
Application
Premixed and prequalified, Protein Extraction Reagent Type 1 is a highly effective protein solubilization and extraction reagent based on conventional extraction formulations. Reducing and alkylating reagents TBP (cat# T7567) and iodoacetamide (cat# A3221) are recommended for use with this reagent. Protein Extraction Reagent Type 1 is available as a component of the ProteoPrep® Sample Extraction Kit (PROTTOT).
Components
Contains urea, Trizma® and CHAPS
Legal Information
ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Qing-Yu Fan et al.
Journal of cellular biochemistry, 119(1), 1093-1110 (2017-07-12)
This study aimed to investigate the effects of SPAR signaling pathway on the restoration of motor function in ischemic stroke (IS). Sprague-Dawley male rats were separated into the control and sham groups, as well as the group for middle cerebral
Yuan Wang et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 48(3), 919-933 (2018-07-24)
Chronic compression of the spinal cord causes the loss of motor neurons in the anterior horn, but the precise and extensive mechanism for the loss is not completely determined. Therefore, this study aims to explore the role of microRNA-494 (miR-494)
Mallikarjun Gadwala et al.
Applied biochemistry and biotechnology, 169(4), 1459-1466 (2013-01-15)
The outstanding capability of two-dimensional gel electrophoresis in separating all types of proteins basically depends on the efficiency of sample preparation. Sample preparation is one of the most critical steps in two-dimensional gel electrophoresis. Unfortunately, due to severe solubility, resolution
Andy Lo et al.
Analytica chimica acta, 788, 81-88 (2013-07-13)
Isotope labeling liquid chromatography-mass spectrometry (LC-MS) is a major analytical platform for quantitative proteome analysis. Incorporation of isotopes used to distinguish samples plays a critical role in the success of this strategy. In this work, we optimized and automated a
Yiming Deng et al.
Translational stroke research, 11(5), 950-966 (2020-01-31)
Ischemic stroke is associated with various physiological and pathological processes including neuronal apoptosis. Growth-arrest-specific transcript 5 (GAS5), a long non-coding RNA (lncRNA), has been recently reported to affect ischemic stroke-induced neuron apoptosis, while its mechanisms remain largely undefined. Through in silico
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