B6650

X-GlcA

reagent for selection of recombinant bacterial clones

• Synonym(s): 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt, X-GlcA, X-glucuronide CHA salt
• Empirical Formula (Hill Notation): C₁₄H₁₃BrClNO₇ · C₆H₁₃N
• CAS Number: 114162-64-0
• Molecular Weight: 521.79
• MDL number: MFCD00058543
• UNSPSC Code: 12352200
• PubChem Substance ID: 24891926
• NACRES: NA.85

Properties

• grade: Molecular Biology Reagent; for molecular biology
• sterility: non-sterile
• assay: ≥98% (TLC)
• form: powder
• technique(s): nucleic acid detection: suitable
• solubility: DMF: soluble
• suitability: suitable for β-galactosidase test
• storage temp.: −20°C
• SMILES string: NC1CCCCC1.O[C@@H]2[C@@H](O)[C@@H](O[C@@H]([C@H]2O)C(O)=O)Oc3c[nH]c4ccc(Br)c(Cl)c34
• InChI: 1S/C14H13BrClNO7.C6H13N/c15-4-1-2-5-7(8(4)16)6(3-17-5)23-14-11(20)9(18)10(19)12(24-14)13(21)22;7-6-4-2-1-3-5-6/h1-3,9-12,14,17-20H,(H,21,22);6H,1-5,7H2/t9-,10-,11+,12-,14+;/m0./s1
• InChI key: JXCKZXHCJOVIAV-CYRSAHDMSA-N

Description

General description

X-GlcA is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. X-GlcA is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Application

Suitable for use in selection of recombinant bacterial colonies with the lac⁺ phenotype.
X-GlcA has been used in histochemical staining of root sections for light microscopic observation.

Chromogenic substrate for β-glucuronidase (GUS) gene detection.

Principle

When X-GlcA is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of X-GlcA turn an intense blue color, allowing for easy differentiation between lac+ and lac- colonies.