MilliporeSigma
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AC-6000

Sigma-Aldrich

HighPrep PCR Clean-up System

Synonym(s):
PCR clean up kit

form

liquid

packaging

pkg of 250 mL (AC-60250 (HighPrep PCR (250 mL)))
pkg of 5 mL (AC-60005 (HighPrep PCR (5 mL)))
pkg of 50 mL (AC-60050 (HighPrep PCR (50 mL)))
pkg of 500 mL (AC-60500 (HighPrep PCR (500 mL)))

storage condition

do not freeze

storage temp.

2-8°C

General description

Magnetic beads-based reagent for manual and for automated post-PCR and post-enzymatic reaction clean-up, for NGS library clean-up and amplicon size selection.

The HighPrep PCR Clean-up System is a paramagnetic beads-based post PCR clean-up reagent designed for efficient purification of DNA fragments and for size-specific selection of amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, adapoter ligation, microarrays, and so on. The magnetic beads are used for different library prep chemistries for NGS.

Application

HighPrep PCR Clean-up System may be used in:
  • Post-PCR and post-enzymatic reaction clean-up
  • NGS library preparation
  • Amplicon size selection for NGS
  • Microarrays
  • PCR
  • Sanger sequencing
  • Restriction enzyme digestions, adapter ligations
  • Cloning

Features and Benefits

  • High recovery of >100 bp amplicons
  • Adaptable to high throughput liquid handling systems
  • Stable recovery of amplicons greater than 100bp, predictable and consistent size selection
  • No centrifugation or filtration leads to higher and purer yields

Storage and Stability

14 months when stored as directed

Legal Information

HiPrep is a trademark of Cytiva
HighPrep is a trademark of MagBio Genomics, Inc.

Disclaimer

For Research Use Only. Not for Use in Diagnostic Procedures.

Pictograms

Health hazard

Signal Word

Warning

Hazard Statements

Hazard Classifications

Repr. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Protocols

HighPrep™ PCR Clean Up Kit and Purification System Protocol

HighPrep™ PCR purification magnetic clean-up beads for manual and automated post-PCR, post-enzymatic reaction, NGS library clean-up.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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