IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody binds to all rabbit Igs.
Immunoglobulin G (IgG) is part of the immunoglobulin family and is widely expressed in the serum. It consists of a gamma (γ) heavy chain in the constant (C) region. The monomeric structure of IgG consists of two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity (Fab fragments). The third fragment known as fragment crystallizable (Fc).
purified rabbit IgG
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Western Blotting (1 paper)
Citrullination of antithrombin by PADI4 was analyzed by ELISA using alkaline phosphatase conjugated goat anti-rabbit IgG as the secondary diluted at 1:5000 in 0.05M carbonate/bicarbonate buffer (Ph 9.6).
Goat Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody has been used for ELISA assays at a dilution of 1:5,000.
Protein expression in maize endosperm amyloplast preparations or whole cell lysates were analyzed by alkaline phosphatase-conjugated goat anti-rabbit IgG as the secondary antibody.
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide.
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