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Monoclonal Anti-α-Actinin antibody produced in mouse

clone BM-75.2, ascites fluid

MDL number:

biological source


Quality Level



antibody form

ascites fluid

antibody product type

primary antibodies


BM-75.2, monoclonal

mol wt

antigen 100 kDa


15 mM sodium azide

species reactivity

human, chicken, bovine, mouse


indirect immunofluorescence: 1:200 using cultured chicken fibroblasts
microarray: suitable
western blot: suitable



UniProt accession no.

shipped in

dry ice

storage temp.


Gene Information

human ... ACTN1(87)
mouse ... Actn1(109711)

General description

α-actinin is a 100kD actin binding protein found in muscle as well as non-muscle cells. In smooth muscle, α-actinin is identified in dense bodies and plaques characteristic of tissues whereas it is associated with z-discs that define muscle sarcomas in normal skeletal muscle. Monoclonal anti-α-actinin antibody can be used in immunoblotting to study the membrane anchorage sites and immunochemical identification of α-actinin. It can also be used in immunofluorescence to study the localization of α-actinin in cultured cells and tissues. Mouse anti-α-actinin antibody reacts specifically with chicken fibroblasts. The product has also shown reactivity for mouse and human cells.
The gene encoding α-actinin is mapped to the human chromosome 11q13.2. α-actinin belongs to the spectrin protein superfamily.[02]


cytoskeletal fraction of bovine mammary gland epithelial (BMGE) cells.


Immunofluorescence of cardiomyocytes from neonatal and adult mouse hearts was performed using monoclonal mouse anti-α actinin (clone EA-53) at 1:1000.[47]
May be used in the study of skeletal muscle organization and in studies of the membrane anchorage sites of actin.
Monoclonal anti-α-actinin antibody (diluted 1: 500) can be used in native protein binding assays[69]. It can also be used in western blot, microarray, immunohistochemistry [62] and in Immunofluorescence microscopy[56].

Biochem/physiol Actions

α−actinin regulates muscle contraction and cytoskeletal organization.[02] It promotes cell migration and acts as an actin crosslinker.[98][99] Mutations in the α-actinin gene is observed in heterogeneous hypertrophic cardiomyopathy and in juvenile onset atrial fibrillation.[00][01]


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Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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