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A5044

Sigma-Aldrich

Monoclonal Anti-α-Actinin antibody produced in mouse

clone BM-75.2, ascites fluid

Synonym(s):
Anti-BDPLT15
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

BM-75.2, monoclonal

mol wt

antigen 100 kDa

contains

15 mM sodium azide

species reactivity

human, chicken, bovine, mouse

technique(s)

indirect immunofluorescence: 1:200 using cultured chicken fibroblasts
microarray: suitable
western blot: suitable

isotype

IgM

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... ACTN1(87)
mouse ... Actn1(109711)

General description

α-actinin is a 100kD actin binding protein found in muscle as well as non-muscle cells. In smooth muscle, α-actinin is identified in dense bodies and plaques characteristic of tissues whereas it is associated with z-discs that define muscle sarcomas in normal skeletal muscle. Monoclonal anti-α-actinin antibody can be used in immunoblotting to study the membrane anchorage sites and immunochemical identification of α-actinin. It can also be used in immunofluorescence to study the localization of α-actinin in cultured cells and tissues. Mouse anti-α-actinin antibody reacts specifically with chicken fibroblasts. The product has also shown reactivity for mouse and human cells.
The gene encoding α-actinin is mapped to the human chromosome 11q13.2. α-actinin belongs to the spectrin protein superfamily.[02]

Immunogen

cytoskeletal fraction of bovine mammary gland epithelial (BMGE) cells.

Application

Immunofluorescence of cardiomyocytes from neonatal and adult mouse hearts was performed using monoclonal mouse anti-α actinin (clone EA-53) at 1:1000.[47]
May be used in the study of skeletal muscle organization and in studies of the membrane anchorage sites of actin.
Monoclonal anti-α-actinin antibody (diluted 1: 500) can be used in native protein binding assays[69]. It can also be used in western blot, microarray, immunohistochemistry [62] and in Immunofluorescence microscopy[56].

Biochem/physiol Actions

α−actinin regulates muscle contraction and cytoskeletal organization.[02] It promotes cell migration and acts as an actin crosslinker.[98][99] Mutations in the α-actinin gene is observed in heterogeneous hypertrophic cardiomyopathy and in juvenile onset atrial fibrillation.[00][01]

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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SDS

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