MilliporeSigma
All Photos(1)

P5936

Sigma-Aldrich

Pectolyase from Aspergillus japonicus

greener alternative

suitable for plant cell culture, lyophilized powder

Synonym(s):
PEL1
Enzyme Commission number:
MDL number:
NACRES:
NA.72

form

lyophilized powder

Quality Level

specific activity

≥0.3 unit/mg solid

greener alternative product characteristics

Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

technique(s)

cell culture | plant: suitable

application(s)

agriculture

greener alternative category

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.

Application

Pectolyase P5936 (Pel1) is a natural mixed pectolyase produced by the fungus Aspergillus japonicus used to digest components (endopolygalacturonate and pectin) of plant cell walls. Treatment of cell walls with pectolyase can be used to destablilize the cell walls for organelle isolation or to modify the elasticity of the cell walls.
Pectolyase from Aspergillus japonicas has been used to digest the siliques and also in immunofluorescence experiments.
Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Packaging

100, 250 mg in poly bottle
1 g in poly bottle

Other Notes

Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor.

Unit Definition

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 5.5 at 25 °C.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service