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Pectinesterase from orange peel

greener alternative

lyophilized powder, ≥150 units/mg protein

Pectin pectylhydrolase, Pectin methylesterase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:


lyophilized powder

Quality Level

specific activity

≥150 units/mg protein


Protein, 20-50% biuret

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storage temp.


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General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.


Pectinesterase is used to hydrolyze methyl esters of pectin to pectate and methanol. It contains pectin methylesterase and pectin pectylhydrolase. Product P5400 is from orange peel and comes as a lyophilized powder. It contains (NH4)2SO4 and sodium chloride. It has been used to study the pectin methylesterase gene.
The enzyme from Sigma has been used as a standard during the measurement of pectin methylesterase activity in sea buckthorn juices. It has been used in the hydrolysis of cell wall pectins while studying phyllotaxis in Arabidopsis. It has also been used to catalyze pectin hydrolysis (using an ultrasonic measurement device) for monitoring biological processes.


1000 units in glass bottle

Biochem/physiol Actions

Pectinesterase catalyzes the hydrolysis of the methyl esters of pectin to yield pectate and methanol.

Unit Definition

One unit will release 1.0 microequivalent of acid from pectin per min at pH 7.5 at 30 °C.

Physical form

Contains (NH4)2SO4 and sodium chloride

Analysis Note

Protein determined by biuret.

Storage Class Code

13 - Non Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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