L3790

Lysozyme from chicken egg white

10 mg/mL

• Synonym(s): Mucopeptide N-acetylmuramoylhydrolase, Muramidase
• CAS Number: 12650-88-3
• Enzyme Commission number: 3.2.1.17 (BRENDA, IUBMB)
• MDL number: MFCD00131557
• NACRES: NA.56

Properties

• Quality Level: 200
• form: (Ready to use solution:10 mg/mL lysozyme in 25 mM sodium acetate pH 4.5 with 50% glycerol.)
• mol wt: single-chain 14.3 kDa
• concentration: ≥400000 units/mL; 10 mg/mL
• UniProt accession no.: P00698
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: chicken ... LYZ(396218)

Description

General description

Lysosyme is an enzyme found in egg white that breaks down the cell walls in bacteria enhancing the efficiency of DNA and protein extraction.

Application

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Suitable for the purification of both DNA and protein from bacteria.

Biochem/physiol Actions

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

Suitability

Tested for suitability with CelLytic^™ B in protein extraction from bacteria.

Physical form

Solution in 50% glycerol, 25 mM Sodium Acetate

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK Germany: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US),Eyeshields,Gloves