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94310

Sigma-Aldrich

Uricase from Bacillus fastidiosus

lyophilized, ~9 U/mg

Synonym(s):

Urate oxidase

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About This Item

CAS Number:
9002-12-4
Enzyme Commission number:
1.7.3.3 (BRENDA, IUBMB)
MDL number:
MFCD00082126
UNSPSC Code:
12352204
PubChem Substance ID:
57653596
NACRES:
NA.54

biological source

Bacillus sp. (B. fastidiosus)

form

powder

quality

lyophilized

specific activity

~9 U/mg

storage condition

dry at room temperature

concentration

90—100%

color

white to faint beige

solubility

5 mg/mL, clear, colorless to very faintly yellow (in enzyme dilution buffer)

application(s)

detection

storage temp.

−20°C

SMILES string

N.OCC1OC(C(O)C1OP(O)(=O)OCC2OC(C(O)C2O)N3C=CC(=O)NC3=O)N4C=CC(=O)NC4=O

InChI

1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3

InChI key

VPXDHZMLJOJKOX-UHFFFAOYSA-N

General description

Research area: Cell signaling
Uricase is present in many organisms, such as, archaea, bacteria, and eukaryotes. The Gut microbiome is the major source of uricase, a homotetrameric enzyme containing four identical 34 kDa subunits.

Application

Uricase may be used for the determination of uric acid in biological fluids. It may be helpful in the spectrophotometric determination of purines and purine nucleosides.

Biochem/physiol Actions

Uricase catalyzes the oxidation of uric acid to 5-hydroxyisourate, which subsequently forms (S)-allantoin. It is involved in purine metabolism, urate degradation and (S)-allantoin formation The absence of functional uricase in humans has significant physiological and pathophysiological implications. It has been associated with higher serum uric acid levels, leading to an increased risk of conditions related to uric acid crystallization, such as gout. Uricase has emerged as an important therapeutic target for the treatment of conditions related to hyperuricemia, such as gout.

Unit Definition

1 U corresponds to the amount of enzyme which oxidizes 1 μmole uric acid at pH 9.1 and 37 °C.

Other Notes

For the determination of uric acid in biological fluids; In the spectrophotometric determination of purines and purine nucleosides.

pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Resp. Sens. 1

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

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U. Naegele et al.
Methods of Enzymatic Analysis, 7, 140-140 (1985)
M.H. Jensen et al.
Methods of Enzymatic Analysis, 7, 101-101 (1985)
Patrizia Zaramella et al.
Pediatrics, 131(1), e309-e312 (2012-12-05)
This case report describes a preterm newborn infant who was treated with a single dose of rasburicase for an increase in uric acid level. He died on the third day as a result of complications of hemolysis, which appeared to
Sara Cipriani et al.
Journal of neurochemistry, 123(1), 172-181 (2012-06-08)
Urate is the end product of purine metabolism and a major antioxidant circulating in humans. Recent data link higher levels of urate with a reduced risk of developing Parkinson's disease and with a slower rate of its progression. In this
Chia-Ter Chao et al.
Medical principles and practice : international journal of the Kuwait University, Health Science Centre, 21(5), 498-500 (2012-06-13)
To report a case of huge hepatocellular carcinoma associated with tumor lysis syndrome and its management. A 51-year-old hepatitis B carrier visited our clinic with progressive weight loss over recent months. Abdominal ultrasonography and magnetic resonance imaging revealed a huge
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