92101204

B16-F0 Cell Line from mouse

Properties

• Quality Level: 100
• biological source: mouse skin
• description: Mouse melanoma, producing melanin
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Melanin
• receptors: Not specified
• application(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse melanoma, producing melanin

Cell Line Description

Mouse melanoma which produces melanin.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK Germany: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable