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85707

Supelco

2,5-Dihydroxybenzoic acid

matrix substance for MALDI-MS, >99.0% (HPLC)

Synonym(s):
Gentisic acid, 2,5-DHBA, DHB, Hydroquinonecarboxylic acid
Linear Formula:
(HO)2C6H3CO2H
CAS Number:
Molecular Weight:
154.12
Beilstein:
2209119
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.21

Quality Level

grade

matrix substance for MALDI-MS

assay

>99.0% (HPLC)

form

powder

analyte functional class(es)

polymers

analyte chemical class(es)

glycans, lipids, organic molecules, peptides, proteins

technique(s)

MALDI-MS: suitable

mp

200-207 °C
204-208 °C (lit.)

cation traces

Ba: ≤5 mg/kg
Ca: ≤5 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
K: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Na: ≤5 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

ε (extinction coefficient)

>2000 at 337 nm

SMILES string

OC(=O)c1cc(O)ccc1O

InChI

1S/C7H6O4/c8-4-1-2-6(9)5(3-4)7(10)11/h1-3,8-9H,(H,10,11)

InChI key

WXTMDXOMEHJXQO-UHFFFAOYSA-N

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Application

2,5-Dihydroxybenzoic acid is an ionic liquid matrix used for the analysis of biomolecules using matrix-assisted laser desorption/ionization combined with mass spectrometry.
Most commonly used matrix for carbohydrates. For MALDI-MS of free neutral glycans. Produces [M+Na]+ ion; may show a weaker [M+K]+ ion. Other species may be generated by the addition of the appropriate inorganic salt.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

More Documents

Quotes and Ordering

2,5-Dihydroxybenzoic acid butylamine and other ionic liquid matrixes for enhanced MALDI-MS analysis of biomolecules
Mank M, et al.
Analytical Chemistry, 76(10), 2938-2950 (2004)
Naoki Sato
Plant & cell physiology, 56(10), 1890-1899 (2015-08-16)
Monogalactosyldiacylglycerol (MGDG) is ubiquitous in the photosynthetic membranes of cyanobacteria and chloroplasts. It is synthesized by galactosylation of diacylglycerol (DAG) in the chloroplasts, whereas it is produced by epimerization of monoglucosyldiacylglycerol (GlcDG) in at least several cyanobacteria that have been
Matrix-assisted laser desorption/ionization mass spectrometry with additives to 2,5-di-hydroxy-benzoic acid.
Karas, M., et al.,
Org. Mass Spectrom., 28, 1476-1481 (1993)
F Bochner et al.
Clinical pharmacology and therapeutics, 30(2), 266-275 (1981-08-01)
Single oral doses of aspirin (ASA, 1,500 mg), sodium salicylate (NaSA, 1,500 mg, 1,200 mg), and salicyluric acid (SUA, 500 mg) were given to five subjects. Serial plasma and urine samples were collected for 24 hr (plasma) and up to
B E Cham et al.
Clinical chemistry, 26(1), 111-114 (1980-01-01)
We have developed a specific and sensitive method for the determination of salicylic acid, salicyluric acid, and gentisic acid in urine. Any proteins present are precipitated with methyl cyanide. After centrifugation, an aliquot of the supernate is directly injected into

Articles

Methods and Matrices for Mass Spectrometry of Glycans

Glycosylation is known to have profound influence on various physiochemical, cellular and biological functions of proteins. Alterations in this modification are known to affect the immune system and have been associated with various pathological states such as cancer, rheumatoid arthritis, and inflammatory diseases.

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Protocols

Mass Spectrometry of Glycans

Mass Spectrometry of Glycans

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