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81349

Sigma-Aldrich

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt

free from low molecular weight nucleotides, lyophilized

Synonym(s):

Poly(dI-dC) sodium salt

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About This Item

CAS Number:
118578-37-3
MDL number:
MFCD00147947
UNSPSC Code:
12352106
NACRES:
NA.52

grade

for molecular biology

Quality Level

100

form

powder

quality

lyophilized

mol wt

Mr ~2.5 × 106 (broad molecular weight distribution)
Mr ~2500000

packaging

ampule of ≥10 units A260

storage temp.

−20°C

General description

Poly (2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt is an alternating copolymer that acts as a substrate for DNA methyltransferases.

Application

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt has been used in electrophoretic mobility shift assay (EMSA) of the transcription factor AP-2 Alpha (TFAP2A) and in southwestern blotting of human embryonic kidney cells (HEK293) nuclear extract.
Suitable for
  • non-specific blocking agent in EMSA
  • evaluation of DNA methyltransferases
  • DNA-small molecule or drug interaction studies

Biochem/physiol Actions

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid), poly(dI-dC) is a synthetic DNA substrate. At high salt, Poly(dI-dC) exists in left handed helical conformation and reverts to right-handed form upon decreasing salt. It is used as a non-specific competitor in electrophoretic mobility shift assay (EMSA).

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Repeated probing of Southwestern blots using alkaline phosphatase stripping
Jia Y, et al.
Journal of Chromatography A, 1217(45), 7177-7181 (2010)
TFAP2A is a component of the ZEB1/2 network that regulates TGFB1-induced epithelial to mesenchymal transition
Dimitrova Y, et al.
Biology Direct, 12(1), 8-8 (2017)
An enzymatic method for the determination of dCTP and dGTP in picomole amounts.
L Skoog
European journal of biochemistry, 17(2), 202-208 (1970-12-01)
Optimization of Competitor Poly (dI-dC) Poly (dI-dC) Levels is Advised in DNA-Protein Interaction Studies Involving Enriched Nuclear Proteins
Larouche K, et al.
Biotechniques, 20(3), 439-444 (1996)
Xiaomei Xu et al.
eLife, 9 (2020-08-09)
Local activation and long-range inhibition are mechanisms conserved in self-organizing systems leading to biological patterns. A number of them involve the production by the developing cell of an inhibitory morphogen, but how this cell becomes immune to self-inhibition is rather
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