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Sigma-Aldrich

Atto 488 amine

BioReagent, ≥90% (HPLC), suitable for fluorescence

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About This Item

UNSPSC Code:
12352116
NACRES:
NA.32

product line

BioReagent

Quality Level

assay

≥90% (HPLC)

form

solid

mol wt

Mw 858 g/mol

manufacturer/tradename

ATTO-TEC GmbH

λ

in methanol: water (1:1) (with 0.1% perchloric acid)

UV absorption

λ: 502-508 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Atto 488 is a fluorescent label with excellent water solubility. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high photostability, and very little triplet formation. Thus Atto 488 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more. The fluorescence is excited most efficiently in the range 480 - 515 nm. For instance the 488 nm line of the Argon-Ion laser is very suitable for excitation.

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Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

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Analysis of fluorescent nanostructures in biological systems by means of spectral position determination microscopy (SPDM).
Muller, P., et al. et al.
Current Microscopy Contributions to Advances in Science and Technology, 1, 3-12 (2012)
Monitoring single membrane protein dynamics in a liposome manipulated in solution by the ABELtrap.
Rendler, T., et al.
arXiv, 1102-1102 (2011)
Mohd A Mohd Ridzuan et al.
PloS one, 7(3), e33845-e33845 (2012-04-06)
An actomyosin motor complex assembled below the parasite's plasma membrane drives erythrocyte invasion by Plasmodium falciparum merozoites. The complex is comprised of several proteins including myosin (MyoA), myosin tail domain interacting protein (MTIP) and glideosome associated proteins (GAP) 45 and
Britta Walowski et al.
Analytical and bioanalytical chemistry, 401(8), 2465-2471 (2011-09-14)
Free-flow electrophoresis techniques have been applied for separations in various areas of chemistry and biochemistry. Here we focus on the generation of a free-flow electrophoresis chip and direct monitoring of the separation of different molecules in the separation bed of
Trine Berthing et al.
Small (Weinheim an der Bergstrasse, Germany), 7(5), 640-647 (2011-02-04)
Nanowires (NWs) are attracting more and more interest due to their potential cellular applications, such as delivery of compounds or sensing platforms. Arrays of vertical indium-arsenide (InAs) NWs are interfaced with human embryonic kidney cells and rat embryonic dorsal root

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