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71636

Sigma-Aldrich

Sodium phosphate dibasic

BioUltra, for molecular biology, ≥99.5% (T)

Synonym(s):
sec-Sodium phosphate, Disodium hydrogen phosphate, Disodium phosphate, Sodium hydrogenphosphate
Linear Formula:
Na2HPO4
CAS Number:
Molecular Weight:
141.96
EC Number:
MDL number:
eCl@ss:
38070211
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

product line

BioUltra

Assay

≥99.5% (T)

form

coarse powder
fine crystals
powder

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.001% total nitrogen (N)

loss

≤0.1% loss on drying, 110 °C

pH

8.8-9.4 (25 °C, 0.5 M in H2O)

pKa (25 °C)

(1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)

solubility

H2O: 0.5 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤10 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.2 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤10 mg/kg
Zn: ≤5 mg/kg

λ

0.5 M in H2O

UV absorption

λ: 260 nm Amax: ≤0.025
λ: 280 nm Amax: ≤0.020

SMILES string

[Na+].[Na+].[H]OP([O-])([O-])=O

InChI

1S/2Na.H3O4P/c;;1-5(2,3)4/h;;(H3,1,2,3,4)/q2*+1;/p-2

InChI key

BNIILDVGGAEEIG-UHFFFAOYSA-L

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This Item
S3264S7907RDD038
grade

for molecular biology

grade

for molecular biology

grade

-

grade

-

product line

BioUltra

product line

-

product line

BioXtra

product line

BioReagent, Redi-Dri

assay

≥99.5% (T)

assay

≥98.5% (titration)

assay

≥99.0%

assay

≥99.0%

form

coarse powder, fine crystals

form

powder

form

powder or crystals

form

powder or crystals

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.001% total nitrogen (N)

impurities

-

impurities

≤0.1% Insoluble matter

impurities

-

pH

8.8-9.4 (25 °C, 0.5 M in H2O)

pH

8.9-9.2 (25 °C, 50 g/L in water)

pH

8.0-11.0 (20 °C, 1 M in H2O)

pH

8.9-9.2 (25 °C, 50 g/L in water)

Application

Sodium phosphate dibasic has been used:
  • as a component in phosphate buffer
  • in the preparation of MgCl2 hybridization buffer
  • as a component in SSPE solution for the preparation of small RNA hybridisation buffer

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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